核黄素激酶

但是，在古菌核黄素激酶（EC 2.7.1.161）中，常使用CTP而非ATP作为反应底物，催化如下反应：

CTP + 核黄素 ${\displaystyle \rightleftharpoons }$ CDP + FMN [2]

riboflavin kinase 核黄素激酶
Thermoplasma acidophilum中的核黄素激酶晶体结构[1]
命名 系统命名 缩写
识别码
EC编号	2.7.1.26
CAS号	9032-82-0
数据库
IntEnz	IntEnz浏览
BRENDA	BRENDA入口
ExPASy	NiceZyme浏览
KEGG	KEGG入口
MetaCyc	代谢路径
PRIAM	概述
PDB	RCSB PDB PDBj PDBe PDBsum
基因本体	AmiGO / EGO
搜索 PMC 相关文献 PubMed 相关文献

Riboflavin kinase 核黄素激酶
crystal structure of flavin binding to fad synthetase from thermotoga maritina
鑑定
標誌	Flavokinase
Pfam	PF01687
InterPro	IPR015865
SCOP	1mrz / SUPFAM
現有可用的蛋白結構： Pfam   結構 / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum 結構概要

Riboflavin kinase 核黄素激酶
鑑定
標誌	Riboflavin_kinase
Pfam	PF01687
InterPro	IPR015865
現有可用的蛋白結構： Pfam   結構 / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum 結構概要 PDB PDB 1mrz PDB 1n05 PDB 1n06 PDB 1n07 PDB 1n08 PDB 1nb0 PDB 1nb9 PDB 1p4m PDB 1q9s PDB 1s4m

核黄素激酶（英語：，EC 2.7.1.26）是一个催化以下化学反应的酶：

ATP + 核黄素 ${\displaystyle \rightleftharpoons }$ ADP + FMN

该酶催化的反应的底物为ATP和核黄素，产物是ADP和黄素单核苷酸（FMN）。

核黄素激酶也在许多细菌中发现，具有类似的功能，但存在若干数量的氨基酸不同。

反应

+ XTP → + XDP

Riboflavin is converted into catalytically active cofactors (FAD and FMN) by the actions of riboflavin kinase (EC 2.7.1.26), which converts it into FMN, and FAD synthetase (EC 2.7.7.2), which adenylates FMN to FAD. Eukaryotes usually have two separate enzymes, while most prokaryotes have a single bifunctional protein that can carry out both catalyses, although exceptions occur in both cases. While eukaryotic monofunctional riboflavin kinase is orthologous to the bifunctional prokaryotic enzyme,[3] the monofunctional FAD synthetase differs from its prokaryotic counterpart, and is instead related to the PAPS-reductase family.[4] The bacterial FAD synthetase that is part of the bifunctional enzyme has remote similarity to nucleotidyl transferases and, hence, it may be involved in the adenylylation reaction of FAD synthetases.[5]

This enzyme belongs to the family of transferases, to be specific, those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:riboflavin 5'-phosphotransferase. This enzyme is also called flavokinase. This enzyme participates in riboflavin metabolism.

结构

截止2007年底，这类酶中已有14个三级结构被解决，在PDB的登陆代码为1N05，1N06，1N07，1N08，1NB0，1NB9，1P4M，1Q9S，2P3M，2VBS，2VBT，3CTA，2VBU，2VBV。

参考文献

1. PDB 3CTA; Bonanno, J.B., Rutter, M., Bain, K.T., Mendoza, M., Romero, R., Smith, D., Wasserman, S., Sauder, J.M., Burley, S.K., Almo, S.C. . 2008.
2. Ammelburg M, Hartmann MD, Djuranovic S, Alva V, Koretke KK, Martin J, Sauer G, Truffault V, Zeth K, Lupas AN, Coles M. . Structure. 2007, 12 (12): 1577–90. PMID 18073108. doi:10.1016/j.str.2007.09.027.
3. Osterman AL, Zhang H, Zhou Q, Karthikeyan S. . Biochemistry. 2003, 42 (43): 12532–8. PMID 14580199. doi:10.1021/bi035450t.
4. Galluccio M, Brizio C, Torchetti EM, Ferranti P, Gianazza E, Indiveri C, Barile M. . Protein Expr. Purif. 2007, 52 (1): 175–81. PMID 17049878. doi:10.1016/j.pep.2006.09.002.
5. Srinivasan N, Krupa A, Sandhya K, Jonnalagadda S. . Trends Biochem. Sci. 2003, 28 (1): 9–12. PMID 12517446. doi:10.1016/S0968-0004(02)00009-9.

延伸阅读

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此条目包含有源于Pfam以及InterPro的属于公有领域的文本 IPR015865